Figure 2. Relationship between maximum weight loss during fixation and initial lens weight and post-mortem time. Human lenses, ranging in age from 61 to 74 years, were incubated for up to 44 h in 1.5% paraformaldehyde in PBS and the maximum amount of weight lost was compared with the initial lens wet weight (A) and with the post-mortem (PM) time (B). No trends can be observed for either plot and the average weight loss is 25%±2.3%.