Figure 1. The effect of paraformaldehyde fixation and incubation in phosphate buffered saline on the wet weights of human lenses. The effects of fixation (A) were examined by incubating freshly excised human lenses at room temperature in 1.5% formalin in PBS up to a maximum of 44 days. The lenses were removed at regular intervals, blotted dry, and weighed before being replaced in the fixative. The data shown in (A) are the averages for 14 lenses, aged 61–74 years. The data for the effects of incubation in PBS alone (B) are the averages from six freshly excised human lenses, aged 52–71.