Figure 3. Western blot analysis of manganese superoxide dismutase expression in HLE-B3 cells. Total cell lysates were collected from HLE-B3 cells grown in 0.5% serum and stimulated by exposure to 1 μM 17β-E₂ for 0, 1.5, 3, 6, 12, and 24 h. There was no change in the expression of MnSOD at any of the time points. The time points at 0 and 24* hours did not receive estrogen.