Figure 2. Real-time polymerase chain reaction expression of manganese superoxide dismutase in HLE-B3 cells. HLE-B3 cells were incubated with 1 μM 17β-E₂ for 0, 1.5, 3, 6, 12, and 24 h. The expression of MnSOD mRNA was analyzed by quantitative real-time PCR. According to the two-way ANOVA, there were no statistically significant changes in MnSOD mRNA at any time point after estrogen treatment compared to the control (untreated). Data are expressed as mean±SD values. p<0.05 (n=3) was considered significant.