Figure 1. Reverse transcriptase polymerase chain reaction expression of manganese superoxide dismutase in HLE-B3 cells. Total RNA was extracted from confluent HLE-B3 cells and subjected to RT–PCR for MnSOD. The cDNA product yielded one band at the correct predicted molecular weight (199 bp). The authenticity of PCR products was verified by DNA sequencing and a BLAST search of the sequence (refer to Methods).