Figure 8. Effects of extracellular Ca²⁺ and Mg²⁺ on YO-PRO-1 uptake. Both Ca²⁺ and Mg²⁺ decreased YO-PRO-1 uptake but Ca²⁺ has a more potent effect than Mg²⁺. Ca²⁺ and Mg2+ together have a synergic effect on the decrease in YO-PRO-1 uptake. The double asterisk indicates a p<0.01 compared to PBS and the triple asterisk denotes a p<0.001 compared to PBS.