Figure 4. Effects of BAC on ocular surface cells. BAC induces ATP release in extracellular medium (A). The cells were incubated with 0.002% BAC for 15 min. Supernatants were collected for ATP quantification. BAC induces high YO-PRO-1 uptake in conjunctival (B) and corneal (C) cells. Frame: Cells were pre-incubated with 10 μM BBG for 30 min before 0.001% BAC and YO-PRO-1 incubation. BBG inhibited YO-PRO-1 uptake induced by BAC on both conjunctival (A) and corneal (B) cells. The asterisk indicates a p<0.05 compared to PBS and the triple asterisk denotes a p<0.001 compared to PBS.