Table 1 of Müller, Mol Vis 2008; 14:883-888.

Table 1. Amplification primers for FTL and LIM2.

Gene Target Primer Sequence (5’-3’) of primers Ta (°C) Product size (bp)
FTL 337 bp before start codon, exon 1, and intron 1 FTL_1_F CAGCTCGGATTGGTCAATAG 58 725


FTL_1_R CGCTGGTTTTGCATCTTC


Exon 2, intron 2, and exon 3 FTL_2_F GCAGCCTTTGTCTCGTTG 58 689


FTL_2_R AAACCTGCCCGATTAAATTC


Intron 3, exon 4, and 315 bp after stop codon FTL_3_F GGATCCCCATGTAAGTACCC 58 594


FTL_3_R TAGGCCTTCCAGAACAACAG

LIM2 5’UTR, exon 1, and exon 2 LIM2_1_F GGAGGCTTAAGGGATTTGG 58 780


LIM2_1_R TCATGCCAGGAAATGTCAC


Exon 3 LIM2_2_F CACCCATTAGCAAACCAAAC 58 599


LIM2_2_R GAGAAGAAACACCCCAGAAAG


Exon 4 LIM2_3_F TACACAGCACTGGCCTGAC 58 645


LIM2_3_R AGTGGCCCCATTCTAACTTC


Exon 5 and 3’UTR LIM2_4_F TGAGCCAGAAGACAGACTCC 58 667


LIM2_4_R AGAATTTGACCTATACATCTGTTTCC


cDNA LIM2_F AGGCTCCAGTCCCTTCCTC 59 660


LIM2_R CTCCCCCTCCTTTTCAGTG

PCR primers, their product size, and annealing temperature (Ta) for the amplification of the genomic sequence of canine FTL and for the amplification of the genomic and cDNA sequence of canine LIM2 are presented.

Müller, Mol Vis 2008; 14:883-888. http://www.molvis.org/molvis/v14/a106

©2008 Molecular Vision http://www.molvis.org/molvis/

ISSN 1090-0535