Table 1 of
Brand, Mol Vis 2007;
13:920-932.Table 1 of
Brand, Mol Vis 2007;
13:920-932.
Table 1. Quality data of the GeneChip® experiments
The quality of each chip is evaluated and depends (among others) on 3'/5' ratios, the background and scalar factor. The percentage of present calls is also an important indicator of quality. Shown are the mean values of six arrays per treatment group with corresponding standard deviation (SD). All values met the expected quality criteria.
Array 30 min Array 4 h Array 6+6 h
----------------- ------------ ------------ ------------
GAPDH 3'/5' ratio 0.74±0.03 0.89±0.01 0.79±0.02
Actin 3'/5' ratio 1.53±0.07 1.8±0.08 1.68±0.03
Background 51.13±6.59 80.52±6.94 81.68±18.5
Scalar factor 1.56±0.38 1.02±0.14 1.34±0.08
% present 56.69±1.34 54.45±1.19 52.4±1.44
|