Table 1 of
Johnson, Mol Vis 2007;
13:887-919.
Table 1. Cell-specific primary antibodies and lectin
The primary antigen or lectin, structure(s) they label, host, source, and reference are presented.
Primary antigen or lectin Structure labeled Host Source Dilution References
------------------------------ -------------------- ------ -------------- -------- ----------
Calreticulin ER Rabbit Chemicon 1:100 78,79
Middle wavelength-sensitive Cones Rabbit Kind gift from 1:1000 109
cone arrestin (M-CAr) Cheryl Craft
Cytochrome oxidase subunit IV Mitochondrial inner Mouse Molecular 1:500 40,73,94
(COX IV) membrane system Probes
Kinesin KIF3A Photoreceptor ribbon Mouse BD Biosciences 1:100 71
and synaptic
vesicles
Na+/Ca2+ exchanger isoform 1 Synaptic terminals Rabbit Swant 1:100 81,82
(NCX1)
Middle wavelength-sensitive Cones Rabbit Kind gift from 1:1000 65
opsin (M-opsin) Cheryl Craft
Short wavelength-sensitive Cones Rabbit Kind gift from 1:1000 65
opsin (S-opsin) Cheryl Craft
pan-Plasma membrane Ca2+ Synaptic terminals Mouse Affinity 1:100 81
ATPase (PMCA) Bioreagents
Mitochondrial DNA polymerase-γ Mitochondrial matrix Rabbit Lab Vision 1:500 76
(POLG)
Protein kinase C-α (PKCα) Rod bipolar cells Rabbit Sigma 1:1000 68,69
Rhodopsin (1D4) Rods Mouse Chemicon 1:1000 72
Sarcoplasmic-endoplasmic ER Rabbit Affinity 1:400 80
reticulum Ca2+ ATPase isoform Bioreagents
3 (SERCA3)
Synaptotagmin 1 Photoreceptor and Mouse Chemicon 1:100 70,113
bipolar synaptic
vesicles
pan-Voltage-dependent anion Mitochondrial outer Rabbit Calbiochem 1:1000 74
channel (VDAC) membrane
Vesicular glutamate Photoreceptor and Guinea Chemicon 1:1,000 69
transporter 1 (VGluT1) bipolar cell pig
terminals
Peanut agglutinin (PNA)-Alexa Cone outer segments Molecular 1:50 58,69
Fluor 647 Conjugate and terminals Probes
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