Table 1 of
Johnson, Mol Vis 2007;
13:887-919.
Table 1. Cell-specific primary antibodies and lectin
The primary antigen or lectin, structure(s) they label, host, source, and reference are presented.
Primary antigen or lectin Structure labeled Host Source Dilution References ------------------------------ -------------------- ------ -------------- -------- ---------- Calreticulin ER Rabbit Chemicon 1:100 78,79 Middle wavelength-sensitive Cones Rabbit Kind gift from 1:1000 109 cone arrestin (M-CAr) Cheryl Craft Cytochrome oxidase subunit IV Mitochondrial inner Mouse Molecular 1:500 40,73,94 (COX IV) membrane system Probes Kinesin KIF3A Photoreceptor ribbon Mouse BD Biosciences 1:100 71 and synaptic vesicles Na+/Ca2+ exchanger isoform 1 Synaptic terminals Rabbit Swant 1:100 81,82 (NCX1) Middle wavelength-sensitive Cones Rabbit Kind gift from 1:1000 65 opsin (M-opsin) Cheryl Craft Short wavelength-sensitive Cones Rabbit Kind gift from 1:1000 65 opsin (S-opsin) Cheryl Craft pan-Plasma membrane Ca2+ Synaptic terminals Mouse Affinity 1:100 81 ATPase (PMCA) Bioreagents Mitochondrial DNA polymerase-γ Mitochondrial matrix Rabbit Lab Vision 1:500 76 (POLG) Protein kinase C-α (PKCα) Rod bipolar cells Rabbit Sigma 1:1000 68,69 Rhodopsin (1D4) Rods Mouse Chemicon 1:1000 72 Sarcoplasmic-endoplasmic ER Rabbit Affinity 1:400 80 reticulum Ca2+ ATPase isoform Bioreagents 3 (SERCA3) Synaptotagmin 1 Photoreceptor and Mouse Chemicon 1:100 70,113 bipolar synaptic vesicles pan-Voltage-dependent anion Mitochondrial outer Rabbit Calbiochem 1:1000 74 channel (VDAC) membrane Vesicular glutamate Photoreceptor and Guinea Chemicon 1:1,000 69 transporter 1 (VGluT1) bipolar cell pig terminals Peanut agglutinin (PNA)-Alexa Cone outer segments Molecular 1:50 58,69 Fluor 647 Conjugate and terminals Probes |