Table 2 of
Lopez-Martinez, Mol Vis 2007;
13:862-872.
Table 2. Oligonucleotide primers used for amplification of the optineurin gene
Annealing temperature and time, extension time for each primer pair and the predicted length for each of the PCR DNA products in base pairs (bp) are listed. In the Primer sequence column, F indicates forward and R indicates reverse.
Annealing temperature Extension Amplicon and time time length Exon Primer sequence (5'-3') (°C/s) (s) (bp) ---- ------------------------- ----------- --------- -------- 4 F: GCCAGTGGG TTTGTGGGAC R: TGCAAAGGGATGGCATTTC 60/20 20 320 5 F: CACTTTCCTGGTGTGTGACTCC R: AAACAACATCACAATGGATCG 60/25 20 281 6 F: CCCAGCCTTAGTTTGATCTG R: GGGGAGGCTTTATAGTTTGC 60/20 20 278 7 F: CATATTGTGTTAAATCCCTTGC R: GGTCACAACATTTGACCTCC 60/20 20 198 8 F: AGTCTTTGGAATTTTTCTGATG R: ATGGGTGAACTGTATGGTATC 60/30 30 287 9 F: GCTATTTCTCTTAAAGCCAAAG R: ACTCTCGTGTGTGTGGGTG 60/30 14 205 10 F: GAGGTTTGTTTAATGTCAGATG R: TCAAAGGAGGATAAAATTGC 55/30 30 211 11 F: CACTGCGACGTAAAGGAGC R: GCTGCCCTTCTGACTCAAC 65/30 30 231 12 F: ATATTTTCCCCAGGATTCC R: AACGTTCAACAGTTTCTGTTC 55/30 30 196 13 F: CAGGCAGAATTATTTCAAAAC R: AATACAGTCAGGGCTGGC 60/30 5 260 14 F: ACAGCACTACCTCCTCATCGC R: GATGTGAGCTCTGGGTCCTCC 65/30 20 231 15 F: TCAGTGTTGTCATGTTTCGGG R: TGAAAATCCAGGATCACACG 60/30 30 171 16 F: CCTGCAAAATGGAACTAATGG R: ACATTTACCAACAGTTTTGGG 61/20 15 203 |