Table 2 of
Lopez-Martinez, Mol Vis 2007;
13:862-872.Table 2 of
Lopez-Martinez, Mol Vis 2007;
13:862-872.
Table 2. Oligonucleotide primers used for amplification of the optineurin gene
Annealing temperature and time, extension time for each primer pair and the predicted length for each of the PCR DNA products in base pairs (bp) are listed. In the Primer sequence column, F indicates forward and R indicates reverse.
Annealing
temperature Extension Amplicon
and time time length
Exon Primer sequence (5'-3') (°C/s) (s) (bp)
---- ------------------------- ----------- --------- --------
4 F: GCCAGTGGG TTTGTGGGAC
R: TGCAAAGGGATGGCATTTC 60/20 20 320
5 F: CACTTTCCTGGTGTGTGACTCC
R: AAACAACATCACAATGGATCG 60/25 20 281
6 F: CCCAGCCTTAGTTTGATCTG
R: GGGGAGGCTTTATAGTTTGC 60/20 20 278
7 F: CATATTGTGTTAAATCCCTTGC
R: GGTCACAACATTTGACCTCC 60/20 20 198
8 F: AGTCTTTGGAATTTTTCTGATG
R: ATGGGTGAACTGTATGGTATC 60/30 30 287
9 F: GCTATTTCTCTTAAAGCCAAAG
R: ACTCTCGTGTGTGTGGGTG 60/30 14 205
10 F: GAGGTTTGTTTAATGTCAGATG
R: TCAAAGGAGGATAAAATTGC 55/30 30 211
11 F: CACTGCGACGTAAAGGAGC
R: GCTGCCCTTCTGACTCAAC 65/30 30 231
12 F: ATATTTTCCCCAGGATTCC
R: AACGTTCAACAGTTTCTGTTC 55/30 30 196
13 F: CAGGCAGAATTATTTCAAAAC
R: AATACAGTCAGGGCTGGC 60/30 5 260
14 F: ACAGCACTACCTCCTCATCGC
R: GATGTGAGCTCTGGGTCCTCC 65/30 20 231
15 F: TCAGTGTTGTCATGTTTCGGG
R: TGAAAATCCAGGATCACACG 60/30 30 171
16 F: CCTGCAAAATGGAACTAATGG
R: ACATTTACCAACAGTTTTGGG 61/20 15 203
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