Table 2 of Lopez-Martinez, Mol Vis 2007; 13:862-872.


Table 2. Oligonucleotide primers used for amplification of the optineurin gene

Annealing temperature and time, extension time for each primer pair and the predicted length for each of the PCR DNA products in base pairs (bp) are listed. In the Primer sequence column, F indicates forward and R indicates reverse.

                                    Annealing
                                   temperature   Extension   Amplicon
                                    and time       time       length
Exon    Primer sequence (5'-3')      (°C/s)         (s)        (bp)
----   -------------------------   -----------   ---------   --------
4      F: GCCAGTGGG TTTGTGGGAC
       R: TGCAAAGGGATGGCATTTC         60/20         20         320
5      F: CACTTTCCTGGTGTGTGACTCC
       R: AAACAACATCACAATGGATCG       60/25         20         281
6      F: CCCAGCCTTAGTTTGATCTG
       R: GGGGAGGCTTTATAGTTTGC        60/20         20         278
7      F: CATATTGTGTTAAATCCCTTGC
       R: GGTCACAACATTTGACCTCC        60/20         20         198
8      F: AGTCTTTGGAATTTTTCTGATG
       R: ATGGGTGAACTGTATGGTATC       60/30         30         287
9      F: GCTATTTCTCTTAAAGCCAAAG
       R: ACTCTCGTGTGTGTGGGTG         60/30         14         205
10     F: GAGGTTTGTTTAATGTCAGATG
       R: TCAAAGGAGGATAAAATTGC        55/30         30         211
11     F: CACTGCGACGTAAAGGAGC
       R: GCTGCCCTTCTGACTCAAC         65/30         30         231
12     F: ATATTTTCCCCAGGATTCC
       R: AACGTTCAACAGTTTCTGTTC       55/30         30         196
13     F: CAGGCAGAATTATTTCAAAAC
       R: AATACAGTCAGGGCTGGC          60/30          5         260
14     F: ACAGCACTACCTCCTCATCGC
       R: GATGTGAGCTCTGGGTCCTCC       65/30         20         231
15     F: TCAGTGTTGTCATGTTTCGGG
       R: TGAAAATCCAGGATCACACG        60/30         30         171
16     F: CCTGCAAAATGGAACTAATGG
       R: ACATTTACCAACAGTTTTGGG       61/20         15         203

Lopez-Martinez, Mol Vis 2007; 13:862-872 <http://www.molvis.org/molvis/v13/a94/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535