Table 1 of
Lopez-Martinez, Mol Vis 2007;
13:862-872.Table 1 of
Lopez-Martinez, Mol Vis 2007;
13:862-872.
Table 1. Oligonucleotide primers used for amplification of the myocilin gene
Annealing temperature and time, extension time for each primer pair and the predicted length for each of the PCR DNA products in base pairs (bp) are listed. In the Primer sequence column, F indicates forward and R indicates reverse.
Annealing
temperature Amplicon
and time Extension length
Region Primer sequence (5'-3') (°C/s) time (s) (bp)
-------- ------------------------- ----------- --------- --------
Promoter F: TCCAGAAAGCCTGTGAATTTGA
R: AGGCAGGCCAGAAGCAGC 61.5/20 60 1117
Exon I F: CTCACCAAGCCTCTGCAATG
R: TGAACTCAGAGTCCCCCCAC 62/20 15 654
Exon II F: ACATAGTCAATCCTTGGGCC
R: CATGAATAAAGACCATGTGG 55/20 20 239
Exon III F: TCTGTGTTTGGAAAGATTATGG
R: CCTGAGCATCTCCTTCTGCC 59/30 40 890
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