Figure 1. Detection of Oct 3/4, Nanog Musashi-1, and CD133 in human retinoblastoma tumors, retinoblastoma cell lines and normal human retina by reverse transcriptase polymerase chain reaction

Human retinoblastoma (RB) tumors and cell lines were examined by RT-PCR as described in Methods. A: Nanog, Oct3/4, and Musashi gene expression in WERI-RB27 and Y79 cell lines. M represents marker; Y represents Y79; W represents WERI-RB27. B: Nanog (N), Oct3/4 (O), and Musashi-1 (M) expression in four human RB tumors (RB1, 2, 3, 4). C: CD133 expression seen in Y79 cells (Y) and RB tumors (1,2,3,4), but not WERI-RB27 cells (W). D: Nanog (N), Oct3/4 (O), Musashi-1 (M) and CD133 (C) expression in normal human retina.