Table 1 of
Li, Mol Vis 2007;
13:813-822.Table 1 of
Li, Mol Vis 2007;
13:813-822.
Table 1. Toll-like receptor1-10 gene expression by Taqman real time PCR analysis
ΔCt of TLR1-10 gene transcripts. ΔCt of each transcript was calculated by subtracting the Ct of β-actin from the Ct of target TLR. N refers to the number of different samples analyzed. Triplicated PCR analysis was carried out for each sample. The data presented is the average ΔCt±SEM. Corneal epithelial cells were collected by laser microdissection. Conjunctival epithelial cells were collected by nitrocellulose paper. ND: nondetectable. NA: data not available due to limited amount of RNA obtained from the samples. The asterisk denotes a p<0.05 when cultured conjunctival cells compared to cultured limbal epithelial cells. The sharp (hash mark) denotes a p<0.05 when cultured conjunctival epithelial cells compared to non-cultured conjunctival epithelial cells collected by nitrocellulose paper.
Corneal Conjunctival Cultured Cultured
epithelial epithelial limbal conjunctival
Gene cells (n=2) cells (n=5) cells (n=6) cells (n=6)
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TLR1 7.64±1.00 6.33±0.54# 9.42±0.58* 12.78±0.58
TLR2 8.17±1.02 7.86±0.65# 7.06±0.72* 12.26±0.72
TLR3 5.55±0.93 4.42±0.59# 11.51±0.66 13.35±0.66
TLR4 NA 5.36±0.63# 12.69±1.78 15.56±3.06
(n=5) (n=4)
TLR5 4.75±0.92 4.91±0.58# 9.12±0.65 10.66±0.53
TLR6 11.40±1.27 13.28±0.80# 16.53±0.90 18.20±0.80
TLR7 NA NA ND ND
TLR8 NA NA ND ND
TLR9 ND 12.53±1.28 14.86±0.21 15.42±1.81
(n=3) (n=4)
TLR10 NA NA ND ND
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