Figure 2. Immunolocalization of toll-like receptors in human corneal, limbal, and conjunctival epithelium

Cryosections of human cornea and conjunctival tissues were incubated with various anti-TLR antibodies and visualized using Alex Fluor 488 conjugated secondary antibodies as described in Methods. Nuclei were stained by DAPI present in the mounting solution. The original pictures were taken at 200X magnification. The insets were taken at 400X magnification.