Figure 1. Western blot analysis of toll-like receptor protein expression in cultured human limbal and conjunctival epithelial cells

Three independent samples of primary cultured limbal and conjunctival epithelial cells isolated from different donor tissues with positive identification of each TLR transcript were analyzed (n=3). Cells were lysed in RIPA buffer and 40 μg of total protein was loaded on each lane. The histogram of western blot analysis shows representative results obtained from all samples analyzed. β-Actin was used as the loading control.