Table 1. Polymerase chain reaction primers and conditions for NRG2 mutation screening

Primers used to obtain the initial PCR amplicons and for subsequent sequencing of the NRG2 gene are listed in this table. Exon 1 was split into three amplicons for PCR and sequencing. Exon 12 was initially amplified by PCR using primers 12AF and 12AR and subsequently sequenced using primers 12AF and 12BF.