Figure 1 of Steele, Mol Vis 2007; 13:764-771.

Figure 1. Topological correspondence of Cav1.3 (α1D) in situ hybridization probes

Voltage-dependent calcium channel pore-forming α1 subunits are composed of a single polypeptide consisting of 4 repeating domains (I-IV) joined by intracellular loops. Each repeat domain consists of 6 transmembrane-spanning regions (1-6) connected by intracellular and extracellular loops. Antisense and sense probe sets were generated using cRNA corresponding to 2 different topological regions of the Cav1.3 (α1D) subunit polypeptide. Probe A corresponded to cRNA encoding an N terminal fragment of the polypeptide, while B corresponded to cRNA encoding a fragment of the variable loop between repeat domains II and III. Probe B encompassed the antigenic peptide (*) used to generate the commercially available antibody used in most published immunohistochemical studies of Cav1.3 (α1D) expression.

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Steele, Mol Vis 2007; 13:764-771 <>
©2007 Molecular Vision <>
ISSN 1090-0535