Figure 4. Anti-ciliary neurotrophic factor western immunoblots of ciliary neurotrophic factor receptor-α immunoprecipitated proteins from lamina cribrosa cell and optic nerve head astrocyte lysates and cell culture medium

PC indicates positive control (recombinant ciliary neurotrophic factor protein). Lane 1 represents LC cells from a 90 year old donor; lane 2 represents optic nerve head astrocyte cells from a 66 year old donor; lane 3 represents ONHA cells from a 90 year old donor.