Figure 9. Vitreous activates AP-1 transcription factor

Nuclear protein extracts from control retinal pigment epithelial (RPE) cells or RPE cells treated with vitreous for one hour were incubated with a biotin-labeled probe, corresponding to the consensus binding sequence for AP-1. The probe-transcription factor complexes were separated from an unbound probe on a 5% polyacrylamide gel, transferred to nylon membranes, and detected by chemiluminescence. 1 represents labeled probe only; 2 represents labeled probe + anti-c-fos antibody; 3 represents control nuclear protein extract + labeled probe; 4 represents control nuclear protein extract + labeled probe + unlabeled probe; 5 represents control nuclear protein extract + labeled probe + anti-c-fos antibody; 6 represents vitreous-treated cell nuclear protein extract + labeled probe; 7 represents vitreous-treated cell nuclear protein extract + labeled probe + unlabeled probe; and 8 represents vitreous-treated cell nuclear protein extract + labeled probe + anti-c-fos antibody. The arrow indicates the position of the probe/AP-1 complex.