Figure 8. Inhibitors of p38 mitogen-activated protein kinase and Jun N-terminal kinase but not of extracellular signal-regulated kinase 1/2 or PI3 kinase inhibit the vitreous-mediated expression of heme oxygenase-1 mRNA

A: Cells were incubated for 12 h with control medium, 25% vitreous or 25% vitreous supplemented with 10 μM SB203580 which inhibits p38 mitogen-activated protein kinase (MAPK). RNA was extracted and heme oxygenase-1 (HO-1) mRNA was measured by qPCR. B: Cells were incubated for 12 h with control medium, 25% vitreous or 25% vitreous supplemented with 50 μM PD98059, which inhibits ERK1/2. C: Cells were incubated for 12 h with control medium, 25% vitreous or 25% vitreous supplemented with 50 μM LY294002, D: Cells were incubated for 12 h with control medium, 25% vitreous or 25% vitreous supplemented with 10 μM SP600125, which inhibits Jun N-terminal kinase (JNK). Ratios show the change in mRNA expression compared to control (no change equal to ratio of 1). Asterisk (*) indicates that REST-XL analysis showed a change that is significantly different from control levels of HO-1 mRNA (p<0.05). Double asterisk (**) denotes a value significantly different from the level of HO-1 mRNA in vitreous-treated cells (p<0.05).