Figure 1 of Geller, Mol Vis 2007; 13:730-739.

Figure 1. Plasmid map of the pFTMGW vector

A: Two primary modifications were made to the parent vector, pFUGW; both are surrounded by rectangles. First, a novel multiple cloning site (MCS) replaced the human ubiquitin-C promoter. Second, a transcription blocker (TB) was cloned immediately upstream of the MCS to prevent aberrant expression from the upstream CMV promoter. B: The sequence (5'-3') of restriction enzyme sites included in the new MCS. ColE1 ori (bacterial origin of replication) and AmpR (ampicillin resistance gene) are used for plasmid replication. The enhanced green fluorescent protein (eGFP) is the reporter molecule. The CMV (cytomegalovirus promoter), LTRs (long terminal repeats), SD/Psi (splice donor and viral packaging sequences), CPPT (central polypurine tract), and WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element) are virus-related elements.

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Geller, Mol Vis 2007; 13:730-739 <>
©2007 Molecular Vision <>
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