Figure 1. Expression of markers of EMT in LEC

A 1 mm scratch was created in confluent LEC and allowed to heal before real-time RT-PCR was used to determine expression. Lumican is upregulated early in EMT and expression peaks by 2 h before returning to basal expression by 4 h. Expression of the Snail in LEC peaks by 4 h and Slug expression peaks by 8 h. Both Snail and Slug return to basal expression by 24 h.