Table 1 of
Kumar, Mol Vis 2007;
13:667-676.Table 1 of
Kumar, Mol Vis 2007;
13:667-676.
Table 1. Details of primer sets used in the PCR-SSCP analysis of the CYP1B1, MYOC, OPTN, and OPTC genes.
Note that primer sets CY2AF/CY2AR, OPTN4aF/OPTN4aR, and OPTN7F/OPTN7R work with 5% DMSO and 1.5 mM magnesium chloride. Primer sets CY2BF/CY2BR, CY2CF/CY2CR, CY2DF/CY2DR, and CY2EF/CY2ER work with 5% DMSO and 1 mM magnesium chloride. Primer set TG1BF/TG1DBR works with 1 mM magnesium chloride. The rest of the primer sets work with 1.5 mM magnesium chloride. Exon 1 of CYP1B1, exons 1, 2, and 3 of OPTN, and exon 1 of OPTC were not screened for mutations as they are non-coding. AT, annealing temperature.
Amplicon AT
Gene Exon Primer sequence (5'-3') size (bp) (°C)
------ ---- -------------------------------------- --------- ----
CYP1B1
2 CY2AF:TGTCTCTGCACCCCTGAGTGTCA 264 67
CY2AR:GAGGTGAGCCGCCTGGCCCAC
2 CY2BF:TGCTGAGGCAACGGAGGCGGCA 211 68
CY2BR:TGCACCAGGGCCTGGTGGATGG
2 CY2CF:TCCAGATCCGCCTGGGCAGCTG 262 67
CY2CR:CTCAGCACGTGGCCCTCGAGGA
2 CY2DF:CAGCATGATGCGCAACTTCTTCAC 254 64
CY2DR:GCGCCCGAACTCTTCGTTGTGGC
2 CY2EF:TGCCGCTACAGCCACGACGACC 261 64
CY2ER:GAGGATAAAGGCGTCCATCATGTCG
2 CY2FF:GACAAGTTCTTGAGGCACTGCGAA 267 66
CY2FR:TCAGAGGAGAAAAGACCTGGCCCA
3 CY3AF:GCTCACTTGCTTTTCTCTCTCCAC 255 60
CY3AR:CCACAGTGTCCTTGGGAATGTGGTA
3 CY3BF:TCACTATTCCTCATGCCACCACTG 277 60
CY3BR:TGAGCCAGGATGGAGATGAAGAGA
3 CY3CF:AAAGGCGGTGCATTGGCGAAGAAC 291 60
CY3CR:TTACTCCTCATCTCCGAAGATGTGA
MYOC
1 TG1AF:AAACCTCTCTGGAGCTCGGGCA 248 60
TG1AR:TATACTGGCATCGGCCACTCTGG
1 TG1BF:GCCTGCCTGGTGTGGGATGTGG 255 64
TG1DBR:GGCAGCCTGGTCCAAGGTCAATTGG
1 TIG1CF:GGAGGCCACCAAAGCTCGACTCA 212 60
TG1CR:TCTCTTCCTCCAGAACTGACTTGTC
1 TG1DF:CCAAACCAGAGAGTTGGAGACTGC 256 62
TG1DR:AGCCATATCACCTGCTGAACTCAGA
2 TG2F:CTCAACATAGTCAATCCTTGGGCCA 244 60
TG2R:GACATGAATAAAGACCACGTGGGCA
3 TIGR1DF:CAAGTATGGTGTGTGGATGCGAGA 205 64
TIGR1R:GCTCCCCGAGTACACCACAGCA
3 TIGR1F:GCCAAGCTTCCGCATGATCATTG 231 60
TIGR1DR:GTGCCAACTGTGTCGATTCTCCA
3 TIGR2DF:CCTTCTAAGGTTCACATACTGCCTAG 270 66
TIGR2DR:AATGGCACCTTTGGCCTCATCGGTG
3 TIGR3F:ACATTGACTTGGCTGTGGATGAAG 267 64
TIGR3R:ATGGGATGGTCAGGGTCTTGCTG
3 TIGR4F:ACCTCAGCAGATGCTACCGTCAA 216 64
TIGR4R:CCATTGCCTGTACAGCTTGGAGG
OPTN
4 OPTN4aF:AATCGCCAATGGGTTTGTGGGA 198 64
OPTN4aR:ACGTGTCCAGGTTTGGGTGGGC
4 OPTN4bF:AGGAGGACAGCCCCAGTGAAAGCAC 193 72
OPTN4bR:AGGGATGGCATTTCTTGCAGGCCCA
5 OPTN5F:CACTTTCCTGGTGTGTGACTCCATC 280 64
OPTN5R:AAAAAACAACATCACAATGGATCGGTC
6 OPTN6F:CAGCCTTAGTTTGATCTGTTCATTCAC 284 60
OPTN6R:CCAGGGGAGGCTTTATAGTTTGCTC
7 OPTN7F:TGGAAGCTTCCTTGGGTTGCATGTC 275 65
OPTN7R:AACATTTGACCTCCGGTGACAAGCA
8 OPTN8F:GGTTACTCTCTTCTTAGTCTTTGGAA 286 60
OPTN8R:GTATCTTAATTATATCTCAGGAAAGCTG
9 OPTN9F:TTCTCTTAAAGCCAAAGAGAAAGTAAC 232 55
OPTN9R:CACAAG ATTTGAATTCAGTGGCTGGA
10 OPTN10F:GTTTAATGTCAGATGATAATTGTACAGA 223 58
OPTN10R:CTTTGTAAAAATGTATATTTCAAAGGAGG
11 OPTN11F:CGTAAAGGAGCATTGTTTATCCTCA 264 60
OPTN11R:CAATCTGTATAAAAAGGCGATTCTCC
12 OPTN12F:GAAGGTTGGGAGGCAAGACTATAAG 224 60
OPTN12R:CAACAGTTTCTGTTCATTACTAGGCTA
13 OPTN13F:CAGGCAGAATTATTTCAAAACCATTTCTAG 252 60
OPTN13R:CAGGGCTGGCCTCGCTCAGCTGG
14 OPTN14F:TGCATTCATCTAGGTACTAAGTTCTG 229 60
OPTN14R:TCTACGGCCATGCTGATGTGAGCT
15 OPTN15F:GTCTGCTCAGTGTTGTCATGTTTCG 243 60
OPTN15R:GAATCCATTGTAGAGAATGAAGTGGAA
16 OPTN16F:CAAGTGAAACAAACACAACTGCCTG 227 60
OPTN16R:CTGACATTTACCAACAGTTTTGGGGA
OPTC
2 OP2aF:CACTCTGGAGAGCCTGTCCCTCAGA 227 64
OP2aR:GAACTTCAAAGGAATCGCCTTCCCTG
2 OP2bF:CAGGAGACAGGGACAGCTTCTCTC 236 64
OP2bR:CTCCCAGTGTCATGCAGGGAATGTA
3 OP3F:TTTGTGCAAAAGCTGGGCTACTGTG 230 60
OP3R:GCCTATGACCTAGGGATATTGCGA
4 OP4F:GCCCCAGAGGCTAAAGAGATCTCC 265 64
OP4R:CAGGGTGGCTGCATATGCCTGC
5 OP5F:AAAGATAGTGTGTTCTGGTTTCTCTC 296 64
OP5R:GTGGTGGAGGTGATAGATAGTGGA
6 OP6F:CCAACAGGACCCACCAGCCTCCTA 209 64
OP6R:CTGCTCCTGGTATCTAACTTCCATCC
7 OP7F:GGCAGAGCCTCTTGGTGAGGCTCA 276 64
OP7R:GGCCCATGCCTGCATGGTCCTTG
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