Figure 6. Expression of TSC-22 mRNA in cultured HCECs

HCECs at 50% confluency were infected with Ad-PLZF or Ad-LacZ at an MOI of 100. The cells were harvested 48 h after infection. The relative expression of TSC-22 mRNA was determined by real-time PCR, and the amount of each mRNA was calculated relative to the amount of GAPDH mRNA in the same samples. The ratio of the sample from Ad-LacZ was set to "1". The asterisk indicates a significant difference (p<0.005) between Ad-PLZF and Ad-LacZ (n=4 each).