Figure 1. Expression of the mRNA of BTB/POZ-zinc finger-containing transcription factors by RT-PCR

A: Ethidium-bromide-stained agarose gels showing PCR products for PLZF, BCL-6, Kaiso, myoneurin, KIAA0441, ZNF278, and GAPDH. Similar results were obtained in two other experiments (35 cycles). Lane 1: Sub-confluent cultured HCECs; Lane 2: 100% confluent cultured HCECs; Lane 3: in vivo normal HCECs. B: Ethidium-bromide-stained agarose gels showing PCR products for PLZF in vivo normal human corneas, ages: 5, 59, 62, 67, and 73 years (40 cycles).