Figure 5 of Wang, Mol Vis 2007; 13:618-625.

Figure 5. Quantification of Δψm in human trabecular meshwork cells by JC-1

A total of 5x105 normal and transfected human trabecular meshwork (HTM) cells with Pro370Leu mutant myocilin plasmid were analyzed by flow cytometry. A: Normal HTM cells. B: HTM cells transfected with Pro370Leu mutant myocilin plasmid. Red fluorescence (Red line) decreased in Pro370Leu-transfected HTM cells. The reduction in the ratio of Red:green JC-1 fluorescence indicates the drop of Δψm.

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