Figure 3. Altered activation of phospho-eIF2α by Pro370Leu mutant myocilin

The phosphorylation of eIF2α was reduced in human trabecular meshwork (HTM) cells transiently transfected with Pro370Leu mutant myocilin plasmid. The total eIF2α protein is shown to demonstrate equal loading of lanes. β-Actin was used as an internal control. The blots are representative of three experiments. The following terms were used: mock, cells transfected with pcDNA3.1(+) expression plasmid; wild, cells transfected with wild-type myocilin expression plasmid; and P370L, cells transfected with Pro370Leu mutant myocilin expression plasmid.