Figure 2. Expression levels of glucose-regulated protein78 protein in human trabecular meshwork cells

Human trabecular meshwork (HTM) cells were transfected with each expression plasmid (pcDNA3.1(+), wild-type, Pro370Leu mutant myocilin plasmids) or were treated with 2.5 μg/ml Tm. Untreated cells served as the control. Western blot was performed with an anti-glucose-regulated protein78 (GRP78) antibody and an anti-β-actin antibody. The blots are representative of three experiments. The following terms were used: mock, cells transfected with pcDNA3.1(+) expression plasmid; wild, cells transfected with wild-type myocilin expression plasmid; and P370L, cells transfected with Pro370Leu mutant myocilin expression plasmid.