Figure 5. Effect of MnSOD overexpression on glucose-induced activation of H-Ras and MAP kinase

Bovine endothelial cells were transfected with MnSOD expression plasmid DNA using Superfect transfection reagent. After transfection, the cells were incubated in 5 mM or 20 mM glucose for 96 h. Activation of (A) H-Ras and (B) MAP kinase was determined by Western blot technique, and the band density was corrected for β-actin band density. Values obtained from the untransfected cells that were incubated in 5 mM glucose were considered to be 100%, and are mean±SD of three different transfection experiments. Please note that the lane in A between 5 mM glucose and 20 mM glucose is one of the lanes in which the molecular weight markers were loaded. 20 mM±SOD cells transfected with MnSOD that are incubated in 20 mM glucose medium. Asterisk (*) marks p<0.05 for 5 mM glucose, and double asterisk (**) indicates p<0.05 compared to 20 mM glucose.