Figure 2 of Fradot, Mol Vis 2007; 13:594-601.


Figure 2. Transcriptional effect of NR2E3 mutations on Gal4 chimeric receptor

A: COS-1 cells were transiently transfected with 100 ng of Gal4-NR2E3LBD wild-type and mutant expression plasmids. Transcriptional activity of a Gal4 responsive reporter gene was measured. B: HeLa cells were transiently transfected with 100 ng of Gal4-NR2E3LBD wild-type and mutant expression plasmids, and transcriptional activity of a Gal4 responsive reporter gene was measured. Normalized values are expressed as relative luciferase activity. C: Expression of Gal4-NR2E3LBD wild-type and mutants in COS-1 transfected cells. D: Electrophoretic mobility shift assay of Gal4 full-length, wild-type (WT), and R385P mutated Gal4-NR2E3LBD using a Gal4 probe. Bound indicates the different shifted bands, and free denotes unbound probe.

(39 K)
(65 K)

Fradot, Mol Vis 2007; 13:594-601 <http://www.molvis.org/molvis/v13/a64/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535