Table 2 of
Dansault, Mol Vis 2007;
13:511-523.Table 2 of
Dansault, Mol Vis 2007;
13:511-523.
Table 2. List of primers used and the PCR product size
For each pair of primers, the amplicon size is indicated in base pairs. F: forward primer; R: reverse primer. Exons 2, 5, 6, 7, 8, 10, and 12 were amplified in two overlapping fragments. In each case, primer pair (.1F and .1R) amplifies the most upstream 5' part of the target genomic DNA, and primer pair (.2F and .2R) amplifies the most downstream 3' part of the target genomic DNA encompassing the specific exonic sequences. Sequences are given in the 5'-3' direction.
Exon Primer Sequences (5'-3') Amplicon
---- ------ ------------------------- --------
1 1F AGGGAACCGTGGCTCGGC 207
1R GGGTGAGGGAAGTGGCTGC
2 2.1F TTATCTCTCACTCTCCAGCC 178
2.1R CTGTTGTTGCTTGAAGACCAC
2.2F AAACTCTCACCAGCAACTCC 197
2.2R GGAGACCTGTCTGAATATTGC
3 3F GGACGTATGCTGTTGAACCAC 166
3R TGAGCCCAAAGCAGCCACCA
4 4F TGCAGCTGCCCGAGGATTAA 133
4R CCGAAGTCCCAGAAAGACCA
5 5.1F CTCTTCTTCCTCTTCACTCTG 163
5.1R CGCTGTGAGCTAGCTCTAC
5.2F CGGTGGTGTCTTTGTCAACG 170
5.2R AGAGGGCGTTGAGAGTGG
5a 5aF CTCTACAGTAAGTTCTCATACC 173
5aR GGAAGTGGACAGAAAACCAC
6 6.1F TGGTTTTCTGTCCACTTCCC 167
6.1R GCACTCCCGCTTATACTGG
6.2F CCGAGAGTAGCGACTCCAG 184
6.2R AGGAGAGAGCATTGGGCTTA
7 7.1F GTGAGCTGAGATGGGTGA 175
7.1R CTTCCGGTCTGCCCGTTC
7.2F ATGGGCGCAGACGGCATG 171
7.2R GACAGGCAAAGGGATGCAC
8 8.1F CCCTTTTGGAGGCTCCAAG 185
8.1R GATGTTCTATTTCTTTGCAGC
8.2F TCCAACGGAGAAGATTCAG 176
8.2R TCTTTGTACTGAAGATGTGGC
9 9F GGAGGTGGGAACCAGTTTGA 195
9R GTGAAACTGCACAGTCTCTC
10 10.1F CTCGACGTAGACACAGTGC 175
10.1R AATTGGTTGGTAGACACTGG
10.2F TCAGAGAAGACAGGCCAGC 153
10.2R CCCGGAGCAAACAGGTTTAA
11 11F GGGCTCTGACTCTCACTCTG 191
11R TTATGCAGGCCACCACCAGC
12 12.1F GCTGTGTGATGTGTTCCTCA 168
12.1R GACTGTTCATGTGTGTCTGC
12.2F CTGCATGCTGCCCACCAG 163
12.2R AAGAGAGATCGCCTCTGTGC
13 13F CATGTCTGTTTCTCAAAGGG 202
13R CCATAGTCACTGACTGAATTAACAC
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