Table 2 of
Kumar, Mol Vis 2007;
13:39-46.Table 2 of
Kumar, Mol Vis 2007;
13:39-46.
Table 2. Primers used for the mutation analysis of the SLC4A11 gene
Primers were designed using genomic DNA and mRNA (GenBank NM_032034) sequences from the UCSC Genome Bioinformatics site. This mRNA does not have exon 1 sequences. Exon 1 was not analyzed as it is a part of the 5'-UTR.
Annealing Amplicon
temperature size
Exon Sequence (5'-3') (°C) (bp)
---- ----------------------------- ----------- --------
2 F: ATTAAGGCTGGCTTCCCCTGCTATG 67 303
R: CCCTGGAGGCTTTTGCCCGACAAG
3&4 F: CCTTCCTGTGTGTGGCACTTTAACAG 67 439
R: ATCACCTCAGCCCCCAGGTAGAGG
5 F: ACCAGGCAGTGACAGCATCTCATAC 67 354
R: GGGTGGTGGGTCAACAGCCCCTC
6 F: GGGGGCGTTGGGAGGGGCTGTTGA 67 201
R: AGGGGACATGGGACACCCAGTTCCAC
7 F: GTCGGGGAGCCCCAGCTCCCTGG 67 254
R: GGACCCCAAGCAGAGGGCGGGTAA
8 F: CCCGGGCAGGGCCTCCTCTGTTTC 67 328
R: GACAGAGCGCCTGTTAGCCCTGTCC
9&10 F: TCGGCGGGGGCATGGGCCGGACA 67 419
R: AGCCCAGGGCCCAGCCCAGCATAC
11 F: TATGCTGGGCTGGGCCCTGGGCTG 67 444
R: GGGCTGAACCAGATCCCAAGCCTTGA
12 F: GGGGCTCAGGGAGGCCTCCCCCA 67 210
R: AGTGCAGAACCTCCCATCTCGGCTG
13 F: TCCAGGGCCTCCCCCTGCCACAC 67 382
R: GGGACAGCAGGTGCATGAGCACAGC
14 F: GAGGGGAGGGGCCGCATGGGTCAA 67 239
R: AGTAGGGGACAGGCTACTGCTATGCC
15 F: GGCGGTGGGTGACGTGGGGTAGC 67 304
R: CTCGTGGACAGAGCCCCACAGCAGA
16 F: CACCGGAGAACAGGTGTGGAGGGTG 65 325
R: GGCCAGAGGCTCCCCACTCCTCAG
17 F: GGAGGAGTGAGGCCCTGTGGACAGG 67 340
R: TGTGGGCGGCAGGGACCGGGTGTG
18 F: GGCGTGGGTGGGGACACAGCCCCA 67 289
R: CAGCCCGCCCATTCTCCACACCTAGA
19 F: TGGGATGGGTGTCCACTGCCTTCTC 67 199
R: GCTCCAGAGCCAGCCTGGGAGGAC
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