Table 2 of
Hunter, Mol Vis 2007;
13:431-442.Table 2 of
Hunter, Mol Vis 2007;
13:431-442.
Table 2. PAX6 primer sequences used for exon scanning
Product
Tm size
Exon Primer (location) Primer sequence (°C) (bp)
---- ------------------- ------------------------- ---- -------
1 PX6-40F (5'UTR) TCAGGCGCAGGAGGAAGTG 59.9 194
PX6-41R (intron 1) TCAGCGGCTGGAGAGTGAG 59.3
2 PX6-42F (intron 1) CTCACTCTCCAGCCGCTGAC 60.1 400
PX6-43R (intron 2) CTCTCCCGGCGTGGCAGTG 63.6
3 PX6-44F (intron 2) GAGAGTCCATGGGCCTGTGC 60.6 200
PX6-45R (intron 3) CTTCTCCCATGTGAACAATGAAG 53.9
4 PX6-84F (intron 3) GCGACTGAGTGGATCCCTTC 58.2 302
PX6-83R (intron 4) CCCTCCAGCCGGACTGC 61
5 PX6-86F (intron 4) CCGCATGGACGTGTGGTCC 61.7 558
PX6-79R (intron 5) GGAGTTATCTTATGTGACTGAC 51.4
5A PX6-50F (intron 5) CTCTCTACAGTAAGTTCTCATAC 49.6 210
PX6-39R (exon 6) CCAGTCTCGTAATACCTGCC 53.7
6 PAX6-34F (exon 5A) GTCCAAGTGCTGGACAATC 51 917
PAX6-29R (intron 6) AACAAGCTCCCAGCCATCCT 53.7
7 PX6-51F (intron 6) GTTGTTCTTTAAGAGAGTGGGTG 53.5 310
PX6-52R (intron 7) CCAGTGGCTGCCTATATGGAG 57.3
8 PX6-53F (intron 7) GCCTCTTTGGGAGGCTCCAAG 60.4 300
PX6-54R (intron 8) CTGGCTAAATATAGCTCTTTGTAC 51.2
9 PAX6-30F (intron 8) GCCACATCTTCAGTACAAAG 49.6 800
PX6-21R (exon 10) GCCTGTCTTCTCTGGTTCC 53.1
10 PAX6-6F (exon 9) GATCTACCTGAAGCAAGAATACAGG 54.9 590
PX6-23R (exon 11) GTGTTTGTGAGGGCTGTGTC 53.7
11 PX6-22F (exon 10) CCCAGTCACATCCCCATCAG 55.8 350
PX6-55R (intron 11) CCCACTCCTCGCTTCTCCG 60.1
12 PX6-61F (intron 11) CGATCATCAGGCTATCATAC 49.9 415
PX6-62R (intron 12) CCAGGAGGTTTCTCTTAAGG 52.2
13 PX6-63F (intron 12) CATAGTCCATGCTTGTCTCTC 52.7 318
PX6-88R (3'UTR) CACAGGACACAACTGCAGAAC 57.4
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