Table 1 of
Hunter, Mol Vis 2007;
13:431-442.Table 1 of
Hunter, Mol Vis 2007;
13:431-442.
Table 1. Primer sequences used to clone PAX6 from a canine retinal cDNA library
Product
Tm size
Primer Location Sequence (°C) (bp)
-------- --------- ------------------------- ---- -------
PAX6-1F exons 4-5 GCAGAACAGTCACAGCGGAGTG 60.4 464
PAX6-3R exon 7 CCGTCTGCGCCCATCTGTTG 61.4
PAX6-4F exon 7 GTCATCAATAAACAGAGTTCTTCGC 54.3 485
PAX6-5R exon 10 GTGTTGCTGGCCTGTCTTCTCTG 60.1
PAX6-6F exon 9 GATCTACCTGAAGCAAGAATACAGG 54.9 380
PAX6-7R exon 12 GGTGTAGGTATCATAACTCCG 51.9
Primers used to clone 5' end of PAX6
PBKIII F vector GGTCGACACTAGTGGATCCAAAG 57.1 550
PAX6-3R exon 7 CCGTCTGCGCCCATCTGTTG 61.4
Primers used to clone 3' end of PAX6
PAX6-6F exon 9 GATCTACCTGAAGCAAGAATACAGG 54.9 600
PBKVI R vector GCTCTCATGAAGATCTCGCCG 57.7
PAX6-27F exon 12 AACAGTCAGCCAATGGGCAC 53.7 200
PBKVI R vector GCTCTCATGAAGATCTCGCCG 57.7
Primers used to amplify alternatively transcribed EXON 5a
PAX6-14F exon 5 CTCGGTGGTGTCTTTGTCAAC 54.2 250
PAX6-15R exon 6 CTACTCTCGGTTTACTACCACC 54.7
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