Table 1 of
Ahad, Mol Vis 2007;
13:388-396.Table 1 of
Ahad, Mol Vis 2007;
13:388-396.
Table 1. Primers used in detecting single nucleotide polymorphism in chemokine genes
The single nucleotide polymorphism (SNPs) were detected in 141 patients and 282 controls by sequence specific primers polymerase chain reaction (SSP-PCR) method. SSP-PCR utilizes SSPs with 3'-end mismatches and identifies the presence of specific allelic variants through PCR amplification.
Product
size
SNP Chromosome Identity Primer (5'-3') (bp)
------------------- ----------- --------- ------------------------------- -------
MCP-1 -2518A/G 17q11.2-q12 rs1024611 F: AAGTGGGAGGCAGACAGCTA/G 252
R: CTGATAAAGCCACAATCCAGAG
RANTES -403G/A 17q11.2-q12 rs2107538 F: CATGGATGAGGGAAAGGAGG/A 285
R: GAGTCTCTGTCTCTCCCTCA
RANTES -28C/G 17q11.2-q12 rs2280788 F: GCCCTTTATAGGGCCAGTTG/C 314
R: GTCCTAACTGCCACTCCTTG
CCR2 V64I 3p21 rs1799864 F: TTTTTGCAGTTTATTAAGATGAGGAC/T 808
R: GAAGGCAGAAGGTGAATAGTTC
CCR5 -59029G/A 3p21 rs2040388 F: ACTTCACATTAACCCTGTGC/T 712
R: ACTGTCATTCAGCCCAATACC
CCR5 32 bp deletion 3p21 F: GCTCTCATTTTCCATACAGTCAG/A 827/808
R: TATACATAAGGAACTTTCGGAGTG
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