Figure 7. Determination of PDGF-stimulated cell proliferation in Ras or Rac transfected cells by BrdU incorporation assay

DNA synthesis was measured with or without addition of PDGF (1 ng/ml, 60 min) in transient tranfectants: vector (control), Rac N17, Ras N17, and Rac V12. The data are expressed as relative luminescence unit (RLU)/sec with mean±SD (n=12). The results are from 3 separate experiments. Each p value was obtained by using mutant transfectant (with PDGF) against vector transfectant (with PDGF). The asterisk indicates a p<1x10^-7.