Figure 5. Effects of stable expression of the β5 proteasome subunit on the levels of apoptosis in LECs after H₂O₂ treatment

Frequency of apoptotic cells before H₂O₂ treatment was 7.1±1.1% for control LECs (A) and 5.9±0.8% for LECs expressing β5 (B). Twenty-four h after treatment with 40 mM H₂O₂, apoptotic cell frequency increased to 15.7±1.9% in control LECs (C) while LECs overexpressing the β5 proteasome subunit experienced a smaller rise in apoptosis (9.3±0.9%; D). The flow cytometric measurements are representative of three independent experiments.