Table 1 of
Warburton, Mol Vis 2007;
13:318-329.Table 1 of
Warburton, Mol Vis 2007;
13:318-329.
Table 1. Proteins identified in melanolipofuscin granules
MLF proteins were fractioned on a 1D gel. The gel lanes were sliced into sections and proteins were digested and analyzed using automated LC-MSMS and then identified using Mascot. Gel number refers to 3 separate preparations that were analyzed. I represents proteins that were previously identified as components of lipofuscin granules. II represents proteins that were previously identified as part of the melanosome proteome. III represents proteins that were previously identified as part of the macrophage phagosome proteome. IV represents proteins that are preparation contaminants.
Genbank
Gel Accession Tissue
Gel Protein slice number Synonyms Subcellular location specificity
----- ---------------------------------- -------- ---------- ----------------------------------- -------------------- ------------
1,2 acid ceramidaseIII 13 AAC73009 putative heart protein Lysosomal Widely
expressed
1,2,3 actin, betaIII 11, 12, AAH08633
13,
14, 15,
16
2 alpha actinin 4II 6 NP_004915 F-actin cross linking protein Nuclear and Widely
cytoplasmic expressed
1 alpha tubulin, ubiquitous 1, 2, 4, NP_006073
5, 6, 7,
9, 10,
13, 15,
17, 18,
19, 21
3 alpha tubulin 2 9, 10, CAA25855
11,
24
3 alpha tubulin 4I 10 NP_079295
1,2,3 alpha tubulin 6III 1, 5, AAH04949
10,
11, 12,
13, 14,
18, 20,
21, 23,
24
3 ankyrin 1IV 3,4 A35049 Cytoplasmic surface of Erythrocyte
erythrocytic plasma
membrane
1,2,3 annexin A2I 12, 13, AAH23990
14, 15
2 annexin A5III 15, 16 AAH01429
2,3 aspartate aminotransferase 14 NP_002071 transaminase A, Glutamate Mitochondrial
oxaloacetate matrix
transaminase-2
3 ATP Synthase subunit g 24 AAC61597
2,3 ATP Synthase, H+ transporting, 22 AAQ88428
mitochondrial F0 complex,
subunit 6
1,2,3 ATP Synthase, H+ transporting, 18 AAH05960 Mitochondrial
mitochondrial F0 complex,
subunit b
2,3 ATP Synthase, H+ transporting, 22 NP_006347
mitochondrial F0 complex,
subunit d
1,2,3 ATP Synthase, H+ transporting, 23 AAH03678
mitochondrial F0 complex,
subunit f
1,2,3 ATP Synthase, H+ transporting, 2, 9, AAP35873
mitochondrial F1 complex, 10
alpha subunit
1,2,3 ATP Synthase, H+ transporting, 10 NP_001677 Widely
mitochondrial F1 complex, beta expressed
subunitII,III
2,3 ATP Synthase, H+ transporting, 16 AAH16812 Mitochondrial
mitochondrial F1 complex,
gamma subunit
1,2,3 ATP Synthase, H+ transporting, 20 AAV38639 Mitochondrial
mitochondrial F1 complex, matrix
subunit o
1,2,3 beta tubulinII,III 10, 11, AAH29529 Ubiquitously
14, 15, expressed
23
3 beta tubulin polypeptide 10, 11, AAH01938
12, 13,
21
1,2,3 calnexinI,II,III 6 I53260 MHC I antigen binding protein p88 Type I membrane
protein
Endoplasmic
reticulum
2,3 cathepsin DI,II,III 15, 16, AAP35556 Lysosomal
17
1,2 cell death-regulatory protein 23 NP_057049 NADH-ubiquinone oxidoreductase Mitochondrial inner Widely
B16.6 expressed
GRIM19 subunit, Gene associated with membrane
retinoic-interferon-induced
mortality 19
protein
2,3 cerebroside sulfate activator 23 AAA36594
protein
2,3 ceroid-lipofuscinosisI,II 12, 13 AAH14863 tripeptidyl-peptidase I precursor, Lysosomal All tissue
lysosomal pepstatin insensitive
protease
1,2,3 chromosome 10 open reading 19, 21 AAQ89418 SFLQ611
frame 58
3 chromosome 10 open reading 24 AAH59168
frame 70
3 chromosome 20 open reading 12 AAQ89435 Type II
membrane protein
frame 3
3 chromosome 8 open reading 13 AAQ88475 Membrane- Ubiquitous
associated
frame 2
2,3 crystallin, alpha BII 22 AAP35416 heat shock protein B5 Lens as well
as other
tissues
2,3 cytochrome c oxidase subunit 22 CAA39187 Integral
III membrane
protein.
Mitochondrial Ubiquitous
inner membrane
2 cytochrome c oxidase subunit 23 AAV38628 Mitochondrial
inner membrane
IV
1 cytokeratin 9IV 1 CAA82315 Terminal
differentiated
epidermis of
palms and
soles
1 epidermal cytokeratin 2IV 1 AAC83410 Epidermal tissue,
squamous metaplasias
and carcinomas
2 erythryocyte membrane protein 7, 8 NP_000110 Membrane- Erythrocyte
band 4.2IV associated and
cytoplasmic
2 gamma glutamyltransferase like 10, 11 NP_004112 Type II membrane
protein
activity 1
2 glucose regulated proteinIII 7 CAB71335 dnaK-type molecular chaperone, BiP Endoplasmic
reticulum
protein, hsp A5 lumen
1 gp25L2 19 CAA62380 Type I membrane
protein
Endoplasmic
reticulum
2,3 guanine nucleotide binding 16 AAA52584 G(o) alpha subunit I
protein
1 guanine-nucleotide binding 13 NP_653082 G protein
protein, alpha transducing
1,2 heat shock 70 kDa protein 9BIII 8, 9 AAH30634 mortalin-2
1 heat shock protein 27 18, 19 AAA62175 estrogen-regulated protein, stress Mitotic spindles in
responsive protein mitotic cells;
nucleus during
heat shock
2 heat shock protein 60III 10 AAA36022 chaperonin 60, mitochondrial matrix Mitochondrial
protein matrix
2,3 heat shock protein gp96 6 AAK74072 tumor rejection antigen, 94kD Endoplasmic
glucose reticulum
regulated protein lumen
1,2,3 hemoglobin beta chainIV 23 AAD19696 Red blood cells
3 hemoglobin, alpha 2IV 24 AAN04486 Red blood cells
3 hemoglobin, beta, mutantIV 24 AAD30656
2 hydroxyacyl dehydrogenase, 8 NP_000173 Long chain 3-hydroxyacyl-CoA Mitochondrial
subunit A dehydrogenase
2,3 hydroxyacyl dehydrogenase, 12 NP_000174
subunit B
1,2,3 hypothetical protein MGC5508 20, 21 NP_076997
1,2,3 keratin 1IV 11 NP_006112.3
2,3 keratin 10IV 16 KRHUO
2 keratin 2aIV 6 NP_000414
1 keratin 6BIV 1 AAH34535 Epithelial in oral
mucosa, esophagus,
papillae of tongue
and hair follicle
2,3 microsomal epoxide hydrolase 12 AAC41694 Membrane-bound Liver
on microsomes
1II
2 microsomal glutathione 23 AAQ81301 Integral Widely
membrane expressed
S-transferase 3 protein.
Microsomal Mitochondrial
2 motor protein 6,7 BAA04654 inner membrane inner
protein, mitochondrial;
membrane
mitofilin,
2 myelin protein zeroI 15, 19 CAA83513
2,3 myosin heavy chain 11II 2 NP_002465 KIAA0866
2,3 myosin heavy chain nonmuscle 2,4 NP_005955
10II
2,3 myosin heavy chain nonmuscle 2 A61231 MYH9
form AIII
3 myosin light chain 3 24 AAA59853
1,2,3 Na+/K+ ATPase alpha chainI,II 4, 5, A26641 Integral Skin and
membrane kidney
protein. 6
2 NAD(P) transhydrogenase 5 CAA90428 Outside the
inner membrane on the mitochondrial
matrix side
3 NADH cytochrome b5 reductase 14, 15 CAA09006 Diaphorase 1 Membrane bound on ER
and
mitochondrial outer
membrane
2 NADH dehydrogenase 14 NP_004535
(ubiquinone) 1 alpha
subcomplex 10
2 NADH dehydrogenase 15, 16 NP_004993
(ubiquinone) 1 alpha
subcomplex 9
1,2,3 NADH dehydrogenase 19 NP_066552
(ubiquinone) flavoprotein 2
1,2 NADH dehydrogenase 6, 7, AAH30833 NADH ubiquinone oxidoreductase Matrix and
8 cytoplasmic
(ubiquinone), Fe-S protein 1I side of
mitochondrial
inner membrane
2,3 NADH dehydrogenase 12, 13 CAH72148
(ubiquinone), Fe-S protein 2
1,2,3 NADH dehydrogenase 17, 18 NP_004542 NADH ubiquinone reductase
(ubiquinone), Fe-S protein 3
1,2 NADH dehydrogenase 19, 20, NP_002487 NADH-coenzyme Q reductase
(ubiquinone), Fe-S protein 8 21
2 NADH dehydrogenase precursor 7, 8 CAA43412 Matrix and
cytoplasmic side of
mitochondrial inner
membrane.
3 NADH ubiquinone oxidoreductase 4 AAH08146 NDUFV1 protein Matrix side of the
mitochondrial inner
membrane
2,3 peptidylprolyl isomerase B 22 CSHUB cyclophilin B
3 peroxiredoxin 1 20 CAI13096 Cytoplasmic
1 peroxiredoxin 2III 21 CAA80269 thioredoxin peroxidase, Cytoplasmic
thio-specific
antioxidant protein
2 peroxiredoxin 3II 19 AAV38810 Mitochondrial
2,3 predicted: similar to RIKEN 12, 15, NP_001017992
cDNA 4732495G21 gene 16
3 prenylcysteine oxidase 1I 10 NP_057381 KIAA0908, PCL1 Lysosomal Ubiquitous
3 progesterone membrane binding 18 NP_006311
protein
1,2,3 progesterone receptor membrane 19 NP_006658 Microsomal; Widely
component 1 membrane-bound expressed
1,2 prohibitinI,III 17 AAS88903
2,3 protein disulfide isomeraseIII 10 S55507 glucose regulated protein 58 kDa, ER Endoplasmic
60 reticulum
precursor lumen
2,3 RAB11BII,III 21 NP_004209 RAS oncogene family
2,3 RAP1BIII 22 AAH78173 RAS oncogene family
2,3 retinal G protein coupled 13, 15, NP_002912 peropsin, RGR Retinal
17 pigment
receptor epithelium
1,2,3 retinal pigment epithelium 2, 6, 7, NP_000320 RPE65 Retinal
pigment
specific proteinI,II 8, 9, epithelium
10,
11, 12,
13, 15,
17, 24
2,3 retinol binding protein 3 4 CAH74045
2 retinol dehydrogenase 11 15 AAH00112 androgen-regulated short-chain Type II membrane
protein.
dehydrogenase/reductase 1 Endoplasmic reticulum.
1,2,3 retinol dehydrogenase 5 15, 16 AAH28298 11-cis retinol dehydrogenase Membrane-associated Retinal
pigment
(11-cis and 9-cis)I,II epithelium
3 ribophorin II 11 AAH02594 KIAA0115, Type I membrane
protein
dolichyl-diphosphooligosaccharide Endoplasmic
protein reticulum
glycosyltransferase 48 kDa subunit
3 ribophorin II precursor 9 B26168 Dolichyl-diphosphooligosaccharide Type I membrane Expressed in
protein protein all tissues
glycosyltransferase 63 kDa subunit Endoplasmic tested
reticulum
2 serum albuminIV 9 CAA23754 Secreted Plasma
2,3 solute carrier family 2, 11 NP_006507 Glucose transporter type 1 Integral membrane
member 1II protein; primarily
at the cell surface
2 solute carrier family 25, 17 NP_005975
member 1
2 solute carrier family 25, 9 NP_003696 Integral membrane Widely
member 12 protein. expressed
Mitochondrial
inner membrane.
2 solute carrier family 25, 9 NP_055066 Integral membrane Widely
member 13 protein. expressed
Mitochondrial
inner membrane.
2 solute carrier family 25, 12, 13 NP_037518
member 24
2 solute carrier family 25, 16 NP_005879
member 3
2 solute carrier family 25, 17 NP_998776
member 3 isoform b
1 solute carrier family 25, 15, 16, NP_001142 ADP/ADT Integral membrane
17 protein.
Mitochondrial
member 4 inner membrane
2,3 solute carrier family 25, 15, 17, NP_001142
18
member 4
2 solute carrier family 25, 10, 14, NP_001143 ADP/ATP carrier protein
18
member 5II
3 solute carrier family 25, 17 AAA36750 ADP.ATP translocase
member 6
2,3 solute carrier family 25, 10, 16 NP_001627
member A6
1,2,3 solute carrier family 4, anion 2, 4, NP_000333
exchanger, member 1 18
1,2,3 spectrin, alphaII 1, 2, NP_003118
4
1,2,3 spectrin, betaII 1, 2 NP_003119.2
1 succinate dehydrogenase 17 NP_002991 succinate-ubiquinone oxidoreductase Mitochondrial inner
iron membrane
comlex, subunit B, iron sulfur sulfur subunit
3 succinate dehydrogenase 9 NP_004159 Mitochondrial inner
complex, subunit AII membrane
1,2,3 ubiquinol-cyctochrome c 19, 20 AAD38242 Mitochondrial inner
reductase, rieske iron-sulfur membrane
protein
2 ubiquinol-cytochrome-c 18 S00680
reductase
2 ubiquinol-cytochrome-c 12, 13 NP_003356 Mitochondrial inner
reductase core protein III membrane
3 ubiquinol-cytochrome-c 12 AAH00484 UQCRC2 Mitochondrial inner
reductase core protein II membrane; matrix
side
1,2,3 vimentinI,II,III 13 AAA61279 beta tubulin, polypeptide Highly
expressed in
fibroblasts,
some expression
in T and B
lymphocytes
1,2,3 voltage dependent anion 15, 16, NP_003365 porin Outer membrane of Heart, liver
18 mitochondria and and skeletal
channel 1III plasma muscle membrane
1,2,3 voltage dependent anion 15 CAH73108 porin
channel 2II
1,2,3 voltage dependent anion 16 NP_005653 Outer mitochondrial Widely
channel 3 membrane expressed
|