Figure 8 of Warburton, Mol Vis 2007; 13:318-329.


Figure 8. Dinitrophenyl immunoblot

Lipofuscin (LF) and melanolipofuscin (MLF) proteins, 1.4 and 4 μg, that had been derivatized with dinitrophenylhydrazine (DNPH) or not (control) were run on SDS-PAGE, transferred to nitrocellulose, and probed with an α-DNP antibody to show the derivatized protein carbonyls, a common product of protein oxidative damage. Shown for comparison and to demonstrate specificity are lanes of bovine serum albumin (BSA), BSA treated with DNPH, and BSA oxidized with hypochlorite then treated with DNPH. Oxidative damage on proteins in LF and MLF granules is both extensive and comparable, though not identical.

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Warburton, Mol Vis 2007; 13:318-329 <http://www.molvis.org/molvis/v13/a35/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
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