Figure 4. The biological pathway of the differentially expressed genes at 24, 48, and 96 h serum deprivation

A: Genes downregulated by serum deprivation (blue color). B: Genes upregulated by serum deprivation (violet color). The pathway was constructed on PathwayAssist software by searching for the shortest path to connect the genes of interest by other genes or cell processes with which they interacted through expression or regulation only. Each of these additional nodes has documented relation to apoptosis. The genes highlighted in blue color are the genes identified by microarray analysis, and the genes in red ovals diamonds color are the potential target genes identified with the aid of PathwayAssist.