Figure 2 of
Reza, Mol Vis 2007;
Figure 2. maf family gene members are mutually regulated during lens development
Chick embryos were electroporated with wild-type empty vector (A-D), L-Maf (E-L), c-Maf (M-T), and Maf B (U-Z2) in the overlying ectoderm at stage 10 and examined at stage 16 by in situ hybridization. Whole-mount and cryosection in situ hybridizations revealed ectopic c-Maf (F and H, red arrows) and MafB (J, red box shown in the inset; L, red arrows) expression by L-Maf overexpression. Note that endogenous MafB is not expressed in lens cells at this stage. Similarly, c-Maf induced MafB (R, red box shown in the inset; T, red arrows) but not L-Maf. On the other hand, MafB downregulated the expression of both L-Maf (V and X, green arrows) and c-Maf (Z and Z2, green arrows). A control experiment with empty vector showed no effect on normal expression of L-Maf (B and D). GFP fluorescence shows the electroporated area (1st and 3rd columns). This figure is representative of at least three independent experiments. For whole mounts, five to six embryos were used each time.