Figure 7 of
Nomura, Mol Vis 2007;
Figure 7. Effect of phorbol 12-myristate 13-acetate on expression and phosphorylation of p38MAPK and Hsp27
A: Subconjunctival fibroblasts were treated with 10 nM phorbol 12-myristate 13-acetate (PMA) for the indicated times. B: For inhibition experiments, we used bisindolylmaleimide I (BIS) or SB203580. Western blot was employed to examine expression and phosphorylation of p38MAPK and Hsp27. Hsp27 phosphorylation was evaluated with anti-phospho-Hsp27 specific antibody (Ser82). The total amount of Hsp27 was determined by reprobing the membrane with an anti-Hsp27 antibody. It can be seen in the β-actin panel that equal amounts of proteins were loaded.