Figure 7 of Nomura, Mol Vis 2007; 13:2320-2327.

Figure 7. Effect of phorbol 12-myristate 13-acetate on expression and phosphorylation of p38MAPK and Hsp27

A: Subconjunctival fibroblasts were treated with 10 nM phorbol 12-myristate 13-acetate (PMA) for the indicated times. B: For inhibition experiments, we used bisindolylmaleimide I (BIS) or SB203580. Western blot was employed to examine expression and phosphorylation of p38MAPK and Hsp27. Hsp27 phosphorylation was evaluated with anti-phospho-Hsp27 specific antibody (Ser82). The total amount of Hsp27 was determined by reprobing the membrane with an anti-Hsp27 antibody. It can be seen in the β-actin panel that equal amounts of proteins were loaded.

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Nomura, Mol Vis 2007; 13:2320-2327 <>
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