Figure 3. Effect of phorbol 12-myristate 13-acetate on lamellipodia formation

Subconjunctival fibroblasts incubated in serum-free medium were initially treated with bisindolylmaleimide I (BIS) or vehicle and then treated with phorbol 12-myristate 13-acetate (PMA). After fixation, the cells were labeled for F-actin, shown in green. Arrows indicate the lamellipodia. The scale bar represents 10 μm.