Figure 2. Effect of phorbol 12-myristate 13-acetate on subconjunctival fibroblast migration

Serum-starved subconjunctival fibroblast cultures were wounded with a micro-pipette tip. The cells were then treated with various concentrations of phorbol 12-myristate 13-acetate (PMA) with or without bisindolylmaleimide I (BIS). The cells were photographed, and the migrated area was measured 16 h after wounding. A: Results from three independent experiments are presented. Asterisk denotes p<0.05. B: Raw images of the cells at each time and concentration of PMA with or without BIS. Angled parallel lines indicate the area occupied by the initial wound. The scale bar represents 130 μm.