Figure 9. Area of RPE two weeks after seeding onto different substrates Cells cultured on older Bruch’s membrane (age > 70) were slightly larger than cells cultured on younger Bruch's membrane (age < 50), but the difference was not statistically significant. Cells cultured onto unmodified retinal pigment epithelium-derived extracellular matrix (RPE-ECM) were larger than cells seeded onto younger or older Bruch’s membrane; cell size was not changed by nitrite treatment of RPE-ECM. Asterisk (*) indicates p<0.01 for unmodified or modified RPE-ECM versus younger or older Bruch’s membrane.