Figure 8 of Sun, Mol Vis 2007; 13:2310-2319.


Figure 8. Comparison of effects of Bruch’s membrane aging and nonenzymatic nitration of retinal pigment epithelium-derived extracellular matrix (RPE-ECM) on ARPE-19 phagocytosis. Average fluorescein isothiocyanate-labeled rod-outer- segments (FITC-ROS) intensity per cell on younger than 50-year-old donor Bruch’s membrane was higher than on older than 70 year-old donor Bruch’s membrane (129.7 ± 34.8 S.D. versus 67.4 ± 4.2 arbitrary units; * = p < 0.01 with student t-test). Mean fluorescence of RPE after ingesting FITC-ROS is higher for cells seeded onto untreated ECM compared to nitrite-treated ECM (126.7 ± 12.8 S.D. versus 60.8 ± 8.2 arbitrary units; * = p < 0.01 with student t-test). Note that the magnitude of 50% reduction effect is the same for aging of human Bruch’s membrane and nonenzymatic nitration of RPE-ECM.