Table 1 of
Murugesan, Mol Vis 2007;
13:2301-2309.Table 1 of
Murugesan, Mol Vis 2007;
13:2301-2309.
Table 1. Effect of temperature on the molar mass, hydrodynamic radius and polydispersity index of Wt-αA and αAG98R proteins
The asterisk indicates that the protein precipitated on incubation at this temperature. The precipitate was removed by centrifugation and analysis was done using the supernatant containing soluble aggregates. "NA" indicates that soluble proteins were found at nanogram levels. Accurate mass measurements become difficult under these conditions, so data was not analyzed.
Molar mass
distribution Hydrodynamic
across peak Average molar radius, Rh Polydispersity
Temperature (g/mol) mass (g/mol) (nm) index
----------- ------------------ ------------- ------------ --------------
Wt-αA
4 °C 10.61e5- 3.73e5 6.94±0.02e5 8.8±0.2 1.053 (0.8%)
25 °C 10.06e5- 3.64e5 6.71±0.02e5 8.5±0.2 1.053 (1%)
37 °C 8.41e5- 3.87e5 5.68±0.02e5 7.9±0.2 1.032 (1%)
45 °C 11.48e5- 4.58e5 6.32±0.03e5 8.1±0.2 1.017 (0.9%)
53 °C 13.89e5- 6.91e5 8.67±0.02e5 9.1±0.2 1.020 (0.6%)
65 °C* 258.50e5- 99.77e5 195.10±0.04e5 25.8±0.6 1.240 (4%)
αAG98R
4 °C 48.28e5- 18.07e5 26.49±0.01e5 15.2±0.3 1.090 (0.9%)
25 °C 173.30e5- 25.90e5 34.11±0.02e5 16.0±0.4 1.157 (2%)
37 °C 346.60e5- 42.14e5 70.83±0.07e5 19.4±0.5 1.158 (2%)
45 °C* 4385.48e5-120.01e5 166.00±0.04e5 30.2±0.7 1.262 (6%)
53 °C* NA NA NA NA
65 °C* NA NA NA NA
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