Figure 7. Auto-induction of epiregulin in HCECs

HCECs were incubated with 10 ng/ml of epiregulin for 0.5, 1, 2, 3, 6, and 12 h, and the relative expression of mRNA was determined by real time PCR. The amount of mRNA was calculated relative to the amount of GAPDH mRNA in the same samples. Asterisks indicate a significant difference from the corresponding control (0 h). P values were calculated by the two-sample t test. Results are mean±SEM of five independent experiments.