Figure 6. Inhibitory effect of AG1478 on the epiregulin induced phosphorylation of ErbB1 (EGFR) and ERK1/2

HCECs were exposed to serial concentrations of AG1478 for 1 h before incubation with 100 ng/ml of epiregulin for 5 min. The cell lysates were subjected to 7.5% SDS-PAGE, and then phosphorylated ErbB1 and ERK1/2 were detected with anti-phosphorylated-ErbB1 or anti-phosphorylated ERK1/2 antibodies. The expression levels of ErbB1 and ERK1/2 were measured relative to that of β-actin in the same sample.